These movies document the growth of the NB cell line CHLA-LAN-5 (top panel) and after treatment with 5uM Retinoic Acid (lower panel) utilizing the Incucyte (Essen). This experiment was performed by Jenna Tong, Post-Bac 2009.
Title: CHLA-LAN-5 neuroblastoma cell growth in vitro
Description: CHLA-LAN-5 cell growth. Note neuroblastoma cells are motile and clumps of cells aggregate. Cells continue to proliferate until the entire bottom of the flask is confluent.
Title: CHLA-LAN-5 neuroblastoma cells treated with retinoic acid in vitro.
Decription: CHLA-LAN-5 cells treated with 5uM all trans-retinoic acid. Note neuroblastoma cells growing in clumps, disperse and begin to extend short extensions. By 48hrs cells have ceased to proliferate and the extension of processes increases. Subsequently cells begin to re-aggregate and the clumps are tethered to each other and the bottom of the flask by longer neuritic-like extensions. In a “tug-of-war” like fashion the clumps of cells move around the flask and aggregate into even larger balls of cells.
For molecular genetic and biochemical studies on ATRA treated cells see our publications:
Thiele CJ, Reynolds CP, Israel MA. Decreased expression of N-myc precedes retinoic acid induced morphological differentiation of human neuroblastoma. Nature 1985;313(6001):404-6.
Thiele CJ, Deutsch LA, Israel MA. The expression of multiple proto-oncogenes is differentially regulated during retinoic acid induced maturation of human neuroblastoma cell lines. Oncogene 1988; 3(3):281-8.
Gaetano C, Matsumoto K, Thiele CJ. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Diff 1991;2:487-93.
Matsumoto K, Gaetano C, Daughaday WH, Thiele CJ. Retinoic acid regulates insulin-like growth factor II expression in a neuroblastoma cell line. Endocrinology 1992;130(6):3669-76.
Gaetano C, Matsumoto K, Thiele CJ. In vitro activation of distinct molecular and cellular phenotypes after induction of differentiation in a human neuroblastoma cell line. Cancer Res 1992;52:4402-7.
Kaplan DL, Matsumoto K, Lucarelli E, Thiele CJ. Induction of trkB by retinoic acid mediates biologic responsiveness to BDNF and differentiation of human neuroblastoma cells. Neuron 1993; 11:1-20.
Cohen P, Matsumoto K, Letterio J, Sporn M, and Thiele CJ. Induction of transforming growth factor 1 (TGFb) during retinoic acid induced differentiation of human neuroblastoma cells. Cancer Res 1995; 55: 2380-2386.
Lucarelli E, Kaplan DR, and Thiele CJ. Selective regualtion of TrkA and TrkB receptor by RA and IFN-gamma in human neuroblastoma cell lines. J Biol Chem 1995; 270:2463-2470.
Giannini G, Dawson MI, Zhang X, and Thiele CJ. Activation of three distinct RXR/RAR heterodimers induces growth arrest and differentiation of Neuroblastoma cells. J. Biol. Chem.1997; 42:26693-26701.
Matsuo T, Thiele CJ. P27Kip1; a key mediator of retinoic acid induced growth arrest in SMS-KCNR human neuroblastoma cells. Oncogene. 1998; 16:3337-3343.
Nakamura M, Matsuo T, Stauffer J, Neckers L, Thiele CJ. Retinoic acid decreases targeting of p27 for degradation via a N-myc dependent decrease in p27 phosphorylation and a N-myc-independent decrease in Skp2. Cell Death and Differ. 2003, 10(2); 230-239.